SoluLinK Product Selection Guide

Instructions:

SoluLinK technology requires two linkers to successfully conjugate 2 biomolecules to give you a quantifiable, controllable and stable result. Determine how to SoluLinK It with the following steps:

Step 1: Select type of conjugation from column A

Step 2: Select the 1^st biomolecule you are working with from column B. Select the reactive group you are working with as well as additional options from column C.  The SoluLinK product you will need appears in column D.

Step 3: Select the 2^nd biomolecule you are working with from column B.  The SoluLinK product you will need appears in column C. Select the reactive group you are working with as well as additional options from column C.  The SoluLinK product you will need appears in column D.

Step 4: Order the products referenced in steps 2 and 3 for a successful conjugation.

A – Type of Conjugate	B- Type of Biomolecule	C- Reactive Group		D – SoluLinK Product
Antibody / Protein	Ab	If using an Amino (NH2) then use:	Linker 1	S-HyNic [S-1002-105]
		If using a Thiol (SH) then use:		MHPH [S-1009-010]
	Protein	If using an Amino (NH2) then use:	Linker 2	S-4FB [S-1004-105]
		If using a Thiol (SH) then use		MTFB [ S-1035-105]
Protein / Oligo	Protein	If using an Amino (NH2) then use:	Linker 1	S-Hynic [S-1002-105]
		If using a Thiol (SH) then use:		MHPH [S-1009-010]
	Oligo	If using a 3’ or 5’ Amino, then use:	Linker 2	S-4FB [S-1004-105]
		If using 4FB-phosphoramidite then use:		4FB Phosphoramidite [S-1005-250] **call
Protein / Peptide	Protein	If using an Amino (NH2) then use:	Linker 1	S-4FB [S-1004-105]
		If using a Thiol (SH) then use:		MTFB [ S-1035-105]
	Peptide	If using N-terminus, then use:	Linker 2	BOC-HyNic [S-3003-100]
		If using C-terminus, then use:		6-FMOC Lysine [S-3034-100]
Oligo / Peptide	Oligo	If using 3’ or 5’ Amino, then use:	Linker 1	S-4FB [S-1004-105] or sulfo-S-4FB [S-1008-105]
		If using 3’ or 5’ Amino, then use:		SS-S-4FB [S-1037-025] cleavable
		If using 4FB-phosphoramidite, then use:		4FB Phosphoramidite [S-1005-250] ** call
	Peptide	If using N-terminus, then use:	Linker 2	BOC-HyNic [S-3003-100]
		If using C-terminus, then use:		6-FMOC Lysine (6-BOC-HyNic) OH [S-3034-100]
Protein / NanoLink Beads Conjugation	NanoLink Beads	If using 4FB NanoLink Beads, then use:	Linker 1	NanoLink Beads [M-1001-010]
	Ab Other protein rPE, APC, perCP HRP, AlkPhos	If using an Amino (NH2), then use:	Linker 2	S-HyNic [S-1002-105]
		If using Thiol (SH), then use:		MHPH [S-1009-010]
NanoLink Beads / Peptide Conjugation	NanoLink Beads	If using 4FB NanoLink Beads, then use:	Linker 1	NanoLink Beads [M-1001-010]
	Peptide	If using N-terminus, then use:	Linker 2	BOC-HyNic [S-3003-100]
		If using C-terminus, then use:		6-FMOC Lysine (6-BOC-HyNic) OH [S-3034-100]