SoluLinK's NanoLink Streptavidin Magnetic Microspheres possess the highest biotin-binding capacity of any commercially available polymer-encapsulated streptavidin particle. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate.

NanoLink microspheres (Catalog # M-1002) are uniform, polymer-encapsulated (no exposed iron), nanometer size, super-paramagnetic particles containing covalently cross-linked streptavidin. Our NanoLink Microspheres are made by covalently cross-linking streptavidin to a hydrophilic surface using SoluLinK’s proprietary aromatic HyNic-4FB conjugation chemistry. The high surface area of these paramagnetic nanoparticles when combined with SoluLinK’s efficient linking chemistry produces a consistent, ultra-high biotin binding capacity per unit mass of polymer microspheres.

The particles are supplied at approximately 1% solids (10 mg/mL) in nuclease-free water with 0.05% sodium azide. There are no surfactants present. Particles can be washed prior to use to remove residual azide, if desired, but it is not necessary for most applications.

Applications include separation of biotin-labeled biomolecules including biotin-labeled genomic DNA, RNA, PCR products, oligonucleotides, peptides, or antibodies. NanoLink Streptavidin Magnetic Microspheres are ideally suited for generating single-stranded PCR templates that can dramatically increase hybridization efficiency to complementary probes by removal of the unbiotinylated competing PCR strand.

The microspheres can be used to immobilize:

• Biotinylated antibodies and other proteins
• Biotinylated dsDNA (gDNA, PCR products) or biotinylated aRNA
• Biotinylated oligonucleotides (>2 nmol/mg binding capacity)

The main featrure of using NanoLink ultra-high biotin-binding microspheres is the reduction in the overall bead mass required to immobilize a biotinylated sample. This provides the benefits of both

• Lower backgrounds of non-specific binding, and
• Lower net costs

Additional Features

• Consistent high biotin binding capacity >8 nmol/mg
• Biotin oligonucleotide binding capacity >2 nmol/mg
• Encapsulated (no exposed iron) monodispersed microspheres
• Uniform size distribution
• Fast magnetic response time (25 electromagnetic units)
• Stable in colloidal form
• Stable in detergents
• Paramagnetic (no residual magnetism)

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