ChromaLink Biotin (DMF Soluble, 10 mg) Product Number:   B-1001-010 Size:   10 mg Price:   $195.00 Quantity:

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Description:
The new ChromaLink Biotin 345S is faster and easier than the traditional and destructive HABA:Avidin displacement biotin measurement method.

Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin 345S from SoluLinK accurately determines the degree of biotin incorporation without any of these disadvantages.

This protein-labeling reagent, also available in an easy-to-use kit (B-9007-105), contains three important features that make it ideal for biotinylation:

1. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling
2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications
3. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems

Highlights:

• Fast and easy method to biotinylate and determine the amount of labeling
• Label and determine the extent of biotin labeling with one reagent
• Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
• Nondestructive assay does not destroy biotinylated sample
• Kit version contains sufficient material to biotinylate 5 - 50 mg of protein and delivers 85% labeled protein recovery with essentially no contamination from non-reacted or hydrolyzed reagent

Applications include:

• Biotinylate protein or peptides
• Quantitate the extent of biotin labeling
• Optimize biotin-avidin complexes

SoluLinK - The Conjugation Company
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San Diego, California 92121