ChromaLink Biotin Labeling Kit - 5 per Kit
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Description:
The ChromaLink Biotin Labeling Kit has everything you need and is faster and easier for labeling and measuring the amount of bound biotin than old traditional HABA:Avidin displacement measurement method. The Kit also has simple protocols on a flash drive and water soluble reagents, so it is safe, no organics needed.
Highlights:
• Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram
• Fast and easy method to biotinylate and determine the amount of labeling
• Label and determine the extent of biotin labeling with one reagent
• Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
• Nondestructive assay does not destroy biotinylated sample
Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin from SoluLinK accurately determines the degree of biotin incorporation without any of these disadvantages.
This reagent, also available as a reagent only in both 5 x 1mg and 10mg formats (B-1007-105 and B-1007-110), contains three important features that make it ideal for biotinylation:
1. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.
2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications.
3. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.
Applications include:
• Biotinylate antibodies, protein or peptides
• Quantitate the extent of biotin labeling
• Optimize biotin-avidin complexes
Other Materials Required:
None  Protocol (PDF)
SoluLinK - The Conjugation Company
9853 Pacific Heights Blvd. Suite H,
San Diego, California 92121
Toll Free: 888.625.0670
Phone: 858.625.0670
Fax: 858.625.0770
solulink@solulink.com
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