ChromaLink Biotin Labeling Kit - 5 per Kit
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Description:
POSTER: Novel multi-color immunofluorescence technique using primary antibodies raised in some host species
The ChromaLink Biotin Labeling Kit has everything you need and is faster and easier for labeling and measuring the amount of bound biotin than old traditional HABA:Avidin displacement measurement method. The Kit also has simple protocols on a flash drive and water soluble reagents, so it is safe, no organics needed.
Highlights:
• Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram
• Fast and easy method to biotinylate and determine the amount of labeling
• Label and determine the extent of biotin labeling with one reagent
• Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
• Nondestructive assay does not destroy biotinylated sample
Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin from SoluLinK accurately determines the degree of biotin incorporation without any of these disadvantages.
This reagent, also available as a reagent only in both 5 x 1mg and 10mg formats (B-1007-105 and B-1007-110), contains three important features that make it ideal for biotinylation:
1. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.
2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications.
3. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.
Applications include:
• Biotinylate antibodies, protein or peptides
• Quantitate the extent of biotin labeling
• Optimize biotin-avidin complex.
Other Materials Required:
none  Protocol (PDF)
Product Data Sheet (PDF)
MSDS (PDF)
SoluLinK - The Conjugation Company
9853 Pacific Heights Blvd. Suite H,
San Diego, California 92121
Toll Free: 888.625.0670
Phone: 858.625.0670
Fax: 858.625.0770
solulink@solulink.com
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