We work with you hand-in-hand to get you the results you need. See the following case studies for examples of what we can do for you. You can also see what our customers have had to say about their experience with Solulink here.
Product used: S-HyNic Conjugation Kit
ImmunoPCR Conjugates: Antibody/Oligo Conjugation
The major hurdle to iPCR chemistry was reliable, efficient and reproducible methods to prepare oligonucleotide/antibody conjugates. In one case a researcher was developing a second generation method that was more sensitive than the methods originally described. Using existing conjugation methods to prepare oligo/antibody conjugates purification was difficult and the yields were low.
We were contracted to prepare multiple antibody/protein conjugates. We delivered antibody/oligo conjugates in `~50% yield, >95% free from unconjugated oligo and antibody. These conjugates performed as required in the assays and many conjugates were subsequently prepared. In many cases only 100 ug of antibody was supplied and the team at Solulink was able to deliver functional conjugates--not a trivial task. A number of papers have been published wherein oligo/antibody conjugates were prepared using HydraLink bioconjugation technology by the researchers themselves or under contract by Solulink.
Peptide/Magnetic Bead Conjugate
A post-doctoral fellow asked us if it was possible to link 40mer peptide to beads using our chemistry. Unfortunately, the answer was no, because using the lysines on a peptide to conjugate it to beads will compromise the epitope. However, she could immobilize the peptide on magnetic beads by having re-synthesizing the peptide with the HyNic linker added on the N-terminus during solid phase synthesis and adding the HyNic-modified peptide to Solulink’s 4FB-NanoLink beads.
Our customer ordered 6-Boc-HNA and synthesized the HyNic peptide was at the institute’s peptide core facility. As advertised, the HyNic-peptide conjugated efficiently to the 4FB-NanoLink beads and the post doc was able to move her research forward within two weeks of ordering 6-Boc-HNA.
SPR and Solulink
A post-doctoral fellow who was studying the kinetics of binding a protein to a peptide told us that he had been trying to link his peptide to a SPR surface using EDC/NHS chemistry, but the immobilization was low-yielding and not reproducible, resulting in poor kinetic data. He hoped to link his peptides to SPR surfaces using without affecting the epitope.
We explained to him that he could accomplish this in three straightforward steps. First have the peptide synthesized with our HyNic linker on the desired terminus. Second, prepare a 4FB surface by modifying an amino surface with sulfo-S-4FB. Finally, expose the 4FB surface to the HyNic peptide at pH 5.0.”
Our customer ordered the HyNic peptide as well the sulfo-S-4FB. Word came back from the post-doc that this worked as advertised, and for the first time he was able to get reproducible kinetic data.